Věra Vávrová Laboratory

Main activities and methods of the laboratory

We are a research team conducting functional studies of rare chronic diseases manifesting in children and adolescents. The subject of the research are hypotheses established by the clinical teams of our department but also by other collaborating clinical and laboratory institutions. We are investigating the mechanisms of disease development and verifying the causality of identified gene variants, e.g. in patients with nephrotic syndrome, growth disorder, osteoporosis and monogenic diabetes. In the laboratory we use classical and state-of-the-art models and methods, e.g. cell and tissue cultures, primary samples from patients, small laboratory animals, mutagenesis, transfection, protein isolation and purification, DNA and RNA sequencing, reverse transcription PCR, western blot, light and fluorescence microscopy, luminescence, absorbance and fluorescence protein essays, differential scanning fluorimetry, microscale thermophoresis and others.