Cardiopulmonary Function in Health and Disease
21–23 September 2006, Prague, Czech Republic
| Organised by: |
The Physiological Society and The Department of Physiology Charles University, Prague, Czech Republic |
| Sponsored by: |
The Physiological Society |
|
Workshop Organisers: |
|
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Professor Vaclav Hampl Department of Physiology 2nd Faculty of Medicine Charles University Plzenska 221/130 Praha 5, 150 06 Czech Republic E-mail: @email |
Dr Sergey V Smirnov Department of Pharmacy and Pharmacology University of Bath Claverton Down Bath BA2 7AY United Kingdom E-mail: @email |
|
Professor Jan Herget Department of Physiology 2nd Faculty of Medicine Charles University Plzenska 221/130 Praha 5, 150 06 Czech Republic E-mail: @email |
Professor Jeremy P.T. Ward Department of Asthma, Allergy and Respiratory Science New Hunt's House King's College London London SE1 9RT E-mail: @email |
Workshop Programme
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Wednesday, September 20th Department of Physiology |
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| 17.00– | Registration |
| 19.00–19.15 | Welcome Speech |
| 19.15– |
Welcome reception (with snacks & drinks) |
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Thursday, September 21st Department of Physiology |
|
| 8.30–9.15 |
Margaret R. MacLean, University of Glasgow, U.K. Pulmonary Hypertension – role of 5-HT |
| 9.15–10.00 |
Jan Herget, Charles University, Prague, Czech Republic The role of radicals in the development of hypoxic pulmonary hypertension |
| 10.00–10.15 |
Tea/coffee break |
| 10.15–12.15 |
Laboratory sessions |
| 12.15–14.00 |
Lunch and Poster session |
| 14.00–16.00 |
Laboratory sessions |
| 16.00–16.15 |
Coffee break |
| 16.15–17.00 |
Keith J. Buckler, University Laboratory of Physiology, Oxford, U.K. Hypoxia, mitochondrial function and chemoreception in the carotid body |
| 17.00–17.45 |
Alison Cave, King's College London, U.K. Auxotonic versus isovolumic contraction: The contribution of cTnI to the positive inotropic response to isoprenaline is highly dependent on loading conditions |
| 18.00– |
Night Prague Sightseeing and pub crawling (with local experts) |
|
Friday, September 22nd Department of Physiology |
|
| 8.30–9.15 |
Philip I Aaronson, King's College London, U.K. Store and receptor operated calcium entry in vascular smooth |
| 9.15–10.00 |
Mark Evans, University of St Andrews, U.K. Multiple stores for multiple messengers: pyridine nucleotides and calcium signalling in pulmonary arterial smooth muscle |
| 10.00–10.15 |
Tea/coffee break |
| 10.15–12.15 |
Laboratory sessions |
| 12.15–14.00 |
Lunch and Poster session |
| 14.00–16.00 |
Laboratory sessions |
| 16.00–16.15 |
Coffee break |
| 16.15–17.00 |
Ivan F. McMutry, University of Colorado, Denver, USA Role of Rho kinase-mediated vasoconstriction in chronic hypoxia-induced pulmonary hypertension |
| 17.00–17.45 |
Jeremy P.T. Ward, King's College London, U.K. Calcium sensitisation and the response of vascular smooth muscle to hypoxia |
| 19.00– |
Workshop Dinner |
|
Saturday, September 23rd Department of Physiology |
|
| 8.30–9.15 |
Vaclav Hampl, Charles University, Prague, Czech Republic The role of NO in hypoxic pulmonary vasoconstriction and pulmonary hypertension |
| 9.15–10.00 |
Jason Yuan, University of California, San Diego, USA Ion Channels in Pulmonary Hypertension |
| 10.00–10.15 |
Tea/coffee break |
| 10.15–12.15 |
Laboratory sessions |
| 12.15–13.00 |
Lunch |
| 14.00–14.45 |
Alison M Gurney, University of Manchester, U.K. Background potassium channels in pulmonary vascular smooth muscle |
| 14.45–15.30 |
Sergey V Smirnov, University of Bath, U.K. Cross-talk between Kv channels and mitochondria in small pulmonary arterial myocytes: The electrophysiological approach |
| 14.45–15.45 |
Best poster presentations and award ceremony |
| 15.45–16.00 |
Concluding Remarks |
|
Sunday, September 24th (Optional) Department of Physiology |
|
| 10.00–12.00 |
– hands-on practical experience (advance signing up requires) |
Demonstrations
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Whole rat pulmonary hemodynamics. Measurements of pulmonary arterial pressure (PAP) by catheterization; cardiac output (CO) by ultrasonic flowmeter on ascending aorta / thermodillution; heart ventricle weights; preparation of lungs for vascular morphology examination.
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Measurements of exhaled NO (anesthetized and awake rats). Measurements of NOx concentration in fluids (e.g. lung perfusate from animals or serum from patients).
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Collection and analysis of breath condensate in rats: measurements of reactive oxygen species (H2O2).
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Isolated ventilated perfused (blood, saline) rat/mouse lungs/hearts. Measurements of vascular reactivity and pressure-flow analysis; on-line pO2 and pCO2 monitoring; demonstration of resistance partitioning by occlusion techniques.
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Isometric tension measurements in isolated resistance arteries with small vessel wire myograph (Mulvany).
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Biochemistry of vascular wall connective tissue (collagen by SDS-PAGE & western, collagenolytic activity by zymography, metalloproteinase expression by immunohistochemistry including computerized image analysis).
Topics included are:
Hypoxic pulmonary vasoconstriction (HPV)
Pulmonary hypertension (PH)
Myocardial ischemia and heart failure
Role of oxidative stress in HPV and PH
Mitochondria and oxygen sensing
Regulation of calcium homeostasis in HPV and PH
Role of ion channels in HPV and PH
Faculty
Philip I. Aaronson, King's College London (UK). Keith J. Buckler, University Laboratory of Physiology, Oxford (UK). Mark Evans, University of St Andrews (UK). Alison M. Gurney, University of Manchester, (UK). Vaclav Hampl, Charles University (Czech republic). Jan Herget, Charles University (Czech republic). Prem Kumar, University of Birmingham (UK). Margaret R. MacLean, University of Glasgow (UK). Michael J. Shattock King's College London (UK). Sergey V. Smirnov, University of Bath (UK). Jeremy P.T. Ward, King's College London (UK). Jason X.-J. Yuan, University of California, San Diego (USA).
