Feasibility of fetal-derived hypermethylated RASSF1A sequence quantification in maternal plasma – next step toward reliable non-invasive prenatal diagnostics.

Abstract:
We determined the feasibility of universal fetal marker detection in maternal circulation. Using real-time PCR, we compared the levels of fetal (SRY and hypermethylated RASSF₁A) and total (GLO gene and total RASSF₁A) extracellular DNA and fractions of extracellular fetal DNA (SRY/GLO vs. hypermethylated RASSF₁A/total RASSF₁A) in maternal circulation. Sensitivity and specificity reached 100% as the fetal-specific hypermethylated RASSF₁A sequence was detected in all 151 examined plasma samples derived from 70 normal pregnancies with a singleton male (n = 51) or female (n = 19) fetus sampled throughout gestation and absent in non-pregnant individuals (n = 29). A strong positive correlation was observed between fetal-derived hypermethylated RASSF₁A and SRY (ρ = 0.66, P < 0.001), total RASSF₁A and GLO (ρ = 0.65, P < 0.001), SRY/GLO vs. hypermethylated RASSF₁A/total RASSF₁A ratio (ρ = 0.62, P < 0.001) in maternal plasma. The results indicate that a universal fetal marker could be useful not only for the confirmation of the presence of fetal cell-free DNA in maternal plasma but could enable quantification of cell-free fetal DNA in pregnancy associated disorders, independently of the sex of the fetus.
 
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