Direct culture-independent Strain typing of Burkholderia cepacia complex in sputum samples from patients with cystic fibrosis.

Drevinek P, Vosahlikova S, Dedeckova K, Cinek O, Mahenthiralingam E. J Clin Microbiol. 2010 May;48(5):1888–91. Epub 2010 Feb 24. IF: 3.945

Abstract:
We examined if multilocus sequence typing (MLST), a method for genotyping and species identification of Burkholderia cepacia complex bacteria, could be applied directly to cystic fibrosis sputum. The redesigned nested-PCR MLST format was successfully used to accurately identify strains in 23 sputum samples, of which 8 were culture negative.
 
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Created: 10. 11. 2010 / Modified: 9. 1. 2019 / prof. MUDr. Radek Špíšek, Ph.D.